| Target: |
TNF alpha (Tumor necrosis factor alpha (TNF-α)) |
| Binding Specificity: |
AA 86-150 |
| Reactivity: |
Human, mouse, rat, pig, rabbit |
| Host: |
Rabbit |
| Clonality: |
Polyclonal |
| Conjugate: |
This TNF alpha antibody is un-conjugated |
| Application: |
ELISA, immunofluorescence (cultured cells) (IF (cc)), immunofluorescence (paraffin-embedded sections) (IF (p)), Immunohistochemistry (frozen sections) (IHC (fro)), immunohistochemistry (paraffin-embedded sections) (IHC (p)), western blotting (WB) |
| Purification: |
Purified by protein A. |
| Immunogen: |
KLH conjugated synthetic peptide derived from human TNF alpha. |
| Isotype: |
IgG |
| Format: |
Liquid |
| Concentration: |
1 μg/μL |
| Buffer: |
0.01 M TBS (pH7.4) with 1% BSA, 0.03% proclin300 and 50% glycerol. |
| Precaution of Use: |
This product contains a poisonous and hazardous substance, which should be handled by trained staff only. |
| Handling Advice: |
Avoid repeated freeze/thaw cycles. |
| Storage: |
- 20°C |
| Storage Comment: |
Store at - 20°C for one year. |
| Expiry Date: |
12 months |
| Alternative Name: |
TNF alpha |
| Synonyms: |
DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa. |
| Background: |
This cytokine binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. Primarily released by macrophages, it triggers cell death in specific tumor cell lines. It acts as a potent pyrogen, inducing fever either directly or by promoting interleukin-1 secretion, and is associated with cachexia induction. Under specific circumstances, it can foster cell proliferation and drive cell differentiation. Additionally, the TNF intracellular domain (ICD) variant stimulates IL12 production in dendritic cells. |
| Molecular Weight: |
17 / 26 kDa |
