| Target: |
TNF alpha (Tumor necrosis factor alpha (TNF-α)) |
| Binding Specificity: |
AA 80-227 |
| Reactivity: |
Mouse |
| Host: |
Rabbit |
| Clonality: |
Polyclonal |
| Conjugate: |
This TNF alpha antibody is un-conjugated |
| Application: |
Western blotting |
| Purpose: |
Rabbit IgG polyclonal antibody for Tumor necrosis factor(TNF) detection. Tested with WB in Mouse. |
| Characteristics: |
Rabbit IgG polyclonal antibody for tumor necrosis factor(TNF) detection. Tested with WB in mouse.
Gene name: tumor necrosis factor.
Protein name: Tumor necrosis factor. |
| Purification: |
Immunogen affinity purified. |
| Immunogen: |
E. coli-derived mouse TNF alpha recombinant protein (Position: L80-Q227). Mouse TNF alpha shares 79% and 95% amino acid (aa) sequence identity with human and rat TNF alpha, respectively. |
| Isotype: |
IgG |
| Format: |
Lyophilized |
| Concentration: |
500 μg/mL |
| Reconstitution: |
Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL. |
| Buffer: |
Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg sodium azide. |
| Precaution of Use: |
This product contains sodium azide: a poisonous and hazardous substance which should be handled by trained staff only. |
| Handling Advice: |
Avoid repeated freezing and thawing. |
| Storage: |
4°C, - 20°C |
| Storage Comment: |
At - 20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at - 20°C for a longer time. |
| Alternative Name: |
TNF alpha |
| Synonyms: |
DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa. |
| Background: |
The TNFα (tumor necrosis factor-alpha) gene encodes a versatile proinflammatory cytokine that is part of the tumor necrosis factor (TNF) superfamily. Macrophages are the primary source of this cytokine's secretion. Its activity is mediated through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNFα plays a pivotal role in regulating numerous biological processes such as cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. |
