| Target: |
TNF alpha (Tumor necrosis factor alpha (TNF-α)) |
| Reactivity: |
Human, mouse, rat |
| Host: |
Rabbit |
| Clonality: |
Polyclonal |
| Conjugate: |
This TNF alpha antibody is un-conjugated |
| Application: |
ELISA, immunocytochemistry (ICC), immunofluorescence (cultured cells) (IF (cc)), immunofluorescence (paraffin-embedded sections) (IF (p)), immunohistochemistry (frozen sections) (IHC (fro)), immunohistochemistry (paraffin-embedded sections) (IHC (p)), western blotting (WB) |
| Purification: |
Purified by protein A. |
| Immunogen: |
Recombinant full length human TNF alpha |
| Isotype: |
IgG |
| Format: |
Liquid |
| Concentration: |
1 μg/μL |
| Buffer: |
0.01 M TBS (pH7.4) with 1% BSA, 0.03% proclin300 and 50% glycerol. |
| Precaution of Use: |
This product contains a poisonous and hazardous substance, which should be handled by trained staff only. |
| Handling Advice: |
Avoid repeated freeze/thaw cycles. |
| Storage: |
- 20°C |
| Storage Comment: |
Store at - 20°C for one year. |
| Expiry Date: |
12 months |
| Alternative Name: |
TNF alpha |
| Synonyms: |
DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa. |
| Background: |
TNF alpha is a protein primarily produced by stimulated macrophages, capable of inducing tumor necrosis in mice. Structurally, it forms multimers but appears as a single 17 kDa band in certain conditions. It shares receptors and cellular effects with Tumour Necrosis Factor beta. TNF alpha can destroy certain transformed cells, especially when combined with interferon-gamma. While it has a limited effect on normal cells, it can directly harm vascular endothelial cells. Additionally, it promotes fibroblast growth, activates immune cells and osteoclasts, and triggers the production of interleukin 1, prostaglandin E2, and collagenase. |
