| Target: |
TNF alpha (Tumor necrosis factor alpha (TNF-α)) |
| Binding Specificity: |
N-term |
| Reactivity: |
Human, mouse, rat, cat, dog, rabbit |
| Host: |
Mouse |
| Clonality: |
Monoclonal |
| Conjugate: |
This TNF alpha antibody is un-conjugated |
| Application: |
Flow cytometry (FACS), immunofluorescence (IF), immunohistochemistry (IHC) |
| Purification: |
PEG precipitation |
| Immunogen: |
An N-terminal recombinant protein fragment was used as the immunogen for the TNFa antibody. |
| Clone: |
TNF706 |
| Isotype: |
IgM kappa |
| Concentration: |
0.2 mg/mL |
| Buffer: |
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA and 0.05% sodium azide. |
| Precaution of Use: |
This product contains sodium azide: a poisonous and hazardous substance which should be handled by trained staff only. |
| Storage: |
4°C, - 20°C |
| Storage Comment: |
Store the TNFa antibody at 2 - 8°C (with azide) or aliquot and store at - 20°C or colder (without azide). |
| Alternative Name: |
TNF alpha |
| Synonyms: |
DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa. |
| Background: |
TNF-alpha is a protein primarily produced by activated macrophages in response to lipopolysaccharides. When injected into mice with tumors, TNF-alpha induces tumor necrosis. It is implicated in the development of pathogenic shock and tissue damage associated with endotoxemia. Structurally, TNF-alpha forms multimers composed of two, three, or five units, yet appears as a singular 17 kDa band in SDS PAGE analysis under non-reducing conditions. Functionally, TNF-alpha shares receptors and cellular effects with Tumor Necrosis Factor Beta (lymphotoxin), a 25 kDa protein. TNF-alpha exhibits cytolytic activity against certain cancerous cells, particularly when combined with interferon-gamma. While it has minimal impact on most normal human cells grown in culture, TNF-alpha can directly harm vascular endothelial cells. Additionally, TNF-alpha stimulates the growth of fibroblasts and various cell lines, activates neutrophils and osteoclasts, and triggers the production of interleukin 1, prostaglandin E2, and collagenase. |
