| Target: |
TNF alpha (Tumor necrosis factor alpha (TNF-α)) |
| Binding Specificity: |
AA 80-235 |
| Reactivity: |
Mouse |
| Host: |
Rabbit |
| Clonality: |
Monoclonal |
| Conjugate: |
This TNF alpha antibody is un-conjugated |
| Application: |
Immunocytochemistry (ICC), immunohistochemistry (IHC), immunoprecipitation (IP), western blotting (WB) |
| Specificity: |
The antibody is a mouse monoclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting. |
| Purpose: |
Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa) |
| Cross-Reactivity: |
Rat |
| Purification: |
Protein A + Protein G affinity chromatography |
| Immunogen: |
Recombinant tumor necrosis factor alpha (TNFa) corresdonding to Leu80~Leu235 with N-terminal His Tag. |
| Clone: |
C1 |
| Isotype: |
IgG1 kappa |
| Format: |
Liquid |
| Concentration: |
Lot specific |
| Buffer: |
0.01 M PBS, pH7.4, containing 0.05% proclin-300, 50% glycerol. |
| Precaution of Use: |
Warning: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Handling Advice: |
Avoid repeated freeze-thaw cycles. |
| Storage: |
4°C, - 20°C |
| Storage Comment: |
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. |
| Expiry Date: |
24 months |
| Alternative Name: |
Tumor necrosis factor alpha |
| Synonyms: |
DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa. |
| Background: |
DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2. |
