| Target: |
TNF alpha (Tumor necrosis factor alpha (TNF-α)) |
| Binding Specificity: |
AA 115-130, N-term |
| Reactivity: |
Human, mouse, rat, cat, dog, rabbit |
| Host: |
Mouse |
| Clonality: |
Monoclonal |
| Conjugate: |
This TNF alpha antibody is un-conjugated |
| Application: |
Flow cytometry (FACS), immunofluorescence (IF), immunohistochemistry (paraffin-embedded sections) (IHC (p)) |
| Purification: |
PEG precipitation |
| Immunogen: |
An N-terminal recombinant protein fragment (TNF706) and amino acids 115-130 (NGVELRDNQLVVPSEG) were used as the immunogen for the TNF-alpha antibody cocktail. |
| Clone: |
TNF706-P-T2 |
| Isotype: |
IgM |
| Concentration: |
1 mg/mL |
| Buffer: |
1 mg/mL in 1X PBS, BSA free, sodium azide free. |
| Storage: |
4°C, - 20°C |
| Storage Comment: |
Store the TNF-alpha antibody cocktail at 2 - 8°C (with azide) or aliquot and store at - 20°C or colder (without azide). |
| Alternative Name: |
TNF alpha |
| Synonyms: |
DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa. |
| Background: |
TNF alpha, produced by macrophages activated by lipopolysaccharides, induces tumor necrosis upon injection into tumor-bearing mice. Structurally, TNF alpha forms multimers of two, three, or five units, yet appears as a single 17 kDa band on SDS PAGE under non-reducing conditions. It triggers cell death in specific transformed cells and exhibits direct toxicity towards vascular endothelial cells while having minimal impact on many normal human cells in culture. Additionally, TNF alpha promotes the growth of human fibroblasts and other cell types, activates immune cells like neutrophils and osteoclasts, and stimulates the production of inflammatory mediators such as interleukin 1, prostaglandin E2, and collagenase. |
