| Target: |
TNF alpha (Tumor necrosis factor alpha (TNF-α)) |
| Binding Specificity: |
AA 115-130 |
| Reactivity: |
Human, mouse, cat, rat, dog, rabbit |
| Host: |
Mouse |
| Clonality: |
Monoclonal |
| Conjugate: |
This TNF alpha antibody is un-conjugated |
| Application: |
Flow cytometry (FACS), immunofluorescence (IF), immunohistochemistry (IHC) |
| Immunogen: |
A hexadecapeptide corresponding to aa115-130 (NGVELRDNQLVVPSEG) of human TNF- , conjugated to thyroglobulin. |
| Clone: |
4C6-H8 |
| Isotype: |
IgM kappa |
| Concentration: |
200 μg/mL |
| Buffer: |
10 mM PBS with 0.05% BSA & 0.05% azide. |
| Precaution of Use: |
This product contains sodium azide: a poisonous and hazardous substance which should be handled by trained staff only. |
| Storage: |
4°C, - 80°C |
| Storage Comment: |
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at - 20 to - 80°C. |
| Expiry Date: |
24 months |
| Alternative Name: |
TNF alpha |
| Synonyms: |
DIF, TNF-alpha, TNFA, TNFSF2, RATTNF, Tnfa, tnf, TNF-a, TNFalpha, Tnfsf1a, TNFa, cTNF, Tnf-alpha, tnfa-like, TNF-ALPHA, dif, tnfa, xtnf, tnfsf2, tnf-alpha, Cachectin, tumor necrosis factor, tumor necrosis factor b (TNF superfamily, member 2), tumor necrosis factor alpha, tumor necrosis factor a (TNF superfamily, member 2), TNF, Tnf, tnf, tnfb, tnf-alpha, LOC103694380, tnfa. |
| Background: |
Tumor necrosis factor alpha (TNF alpha) is a protein secreted by macrophages when stimulated by lipopolysaccharides, and it induces tumor necrosis in tumor-bearing mice upon injection. TNF alpha is implicated in mediating pathogenic shock and tissue injury associated with endotoxemia. Structurally, TNF alpha forms multimers consisting of two, three, or five non-covalently linked units, yet it appears as a single 17 kDa band on SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25 kDa protein tumor necrosis factor beta (lymphotoxin), sharing the same receptors and cellular actions. It induces cytolysis in certain transformed cells and its cytotoxicity is enhanced when combined with interferon-gamma. While TNF alpha has minimal effects on many normal human cells in culture, it is directly toxic to vascular endothelial cells. Additionally, TNF alpha stimulates the growth of human fibroblasts and other cell lines, activates polymorphonuclear neutrophils and osteoclasts, and induces the production of interleukin 1, prostaglandin E2, and collagenase. |
| Molecular Weight: |
17 kDa |
