| Target: |
IL-6 (Interleukin 6) |
| Reactivity: |
Rhesus monkey |
| Detection Method: |
Colorimetric |
| Method Type: |
Sandwich ELISA |
| Detection Range: |
15.625 pg/mL - 1000 pg/mL |
| Minimum Detection Limit: |
15.625 pg/mL |
| Sample Type: |
Serum, plasma, cell culture supernatant, tissue homogenate, cell lysate |
| Analytical Method: |
Quantitative |
| Sensitivity: |
6.4 pg/mL |
| Components: |
Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard diluent
Detection reagent A
Detection reagent B
Assay diluent A
Assay diluent B
TMB
Stop solution
Wash buffer (30X)
Plate sealer for 96 wells
Instruction manual |
| Application: |
ELISA |
| Sample Volume: |
100 μL |
| Assay Time: |
1 - 4.5 h |
| Plate: |
Pre-coated |
| Specificity: |
This assay has high sensitivity and excellent specificity for detection of this index. |
| Purpose: |
The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of IL6 in monkey serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Precaution of Use: |
The stop solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. |
| Handling Advice: |
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end. |
| Storage: |
4°C, - 20°C |
| Storage Comment: |
The assay plate, standard, detection reagent A and detection reagent B should be stored at - 20°C upon being received. After receiving the kit, substrate should be always stored at 4°C. Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at - 20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above. |
| Expiry Date: |
12 months |
| Alternative Name: |
MGI2-A, MGI2A, HGF, BSF2, HSF, IFNB2, B-Cell Stimulatory Factor-2, Hybridoma/Plasmacytoma Growth Factor, Hepatocyte Stimulating Factor, Cytotoxic T-Cell Differentiation Factor |
| Synonyms: |
BSF2, HGF, HSF, IFNB2, IL-6, Il-6, ILg6, Ifnb2, il6, CHIL-6, interleukin 6, interleukin-6, IL6, Il6, il-6, IL-6. |
