| Source: |
Escherichia coli |
| Molecular Weight: |
~52,000 per tetramer. |
| AA Sequence: |
MAEAGITGTW YNQLGSTFIV TAGADGALTG TYESAVGNAE SRYVLTGRYD SAPATDGSGT ALGWTVAWKN NYRNAHSATT WSGQYVGGAE ARINTQWLLT SGTTEANAWK STLVGHDTFT KVKPSAAS |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses |
| Physical Appearance: |
Sterile colorless liquid. |
| Formulation: |
Supplied as a 0.2 μm filtered concentrated sterile solution in 20 mM potassium dihydrogen phosphate buffer, pH6.5. |
| Endotoxin: |
Less than 0.1 EU/μg of rStreptavidin as determined by LAL method. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Refer to lot specific COA for the Use by Date when stored at ≤ -20°C as supplied. |
| Background: |
Streptavidin is a tetrameric protein made up of identical subunits, each of which binds one biotin molecule with an exceptionally high affinity (KD ~1×10-15 M). The preparation includes a core streptavidin variant with shortened N- and C-terminal regions, which offers enhanced properties such as increased homogeneity, solubility, resistance to proteolytic degradation, and improved accessibility of the biotin-binding pocket compared to native streptavidin. |
