| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids. |
| AA Sequence: |
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 × 107 IU/mg in the presence of actinomycin D. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4, 5% trehalose. |
| Endotoxin: |
Less than 1 EU/μg of rRhTNF-α as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
TNFSF2, Cachectin, Differentiation-inducing Factor, DIF, Necrosin, Cytotoxin |
| Background: |
Tumor necrosis factor alpha (TNF-α), also known as cachectin, is a prominent TNF-family member that induces cell death. Produced by a range of cells including neutrophils, activated lymphocytes, macrophages, and others, TNF-α is present in both secreted and membrane-bound forms, both active biologically. The native TNF-α is glycosylated, though its recombinant, non-glycosylated version is similarly active. The active form of native TNF-α is a trimer. TNF-α shares a high amino acid homology between rhesus macaque and humans, around 98%. Two receptor types for TNF-α exist, and most cell types have one or both receptors. |
