| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 10.6 kDa, a single non-glycosylated polypeptide chain containing 91 amino acids |
| AA Sequence: |
SELEKAMVAL IDVFHQYSGR EGDKHKLKKS ELKELINNEL SHFLEEIKEQ EVVDKVMETL DSDGDGECDF QEFMAFVAMV TTACHEFFEH E |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Data Not Available. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rRhS100B as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Protein S100-P, Migration-inducing gene 9 protein, MIG9, Protein S100-E, S100 calcium-binding protein P |
| Background: |
S100B is part of the S100 protein family, characterized by two EF-hand calcium-binding motifs. The S100B protein in humans is encoded by the S100P gene at 4q16, while other S100 family proteins are encoded by genes clustered at 1q21. S100B is glial-specific, primarily expressed by astrocytes, particularly a subtype that ensheaths blood vessels and NG2-expressing cells. It may play roles in neurite extension, melanoma cell proliferation, Ca2+ flux stimulation, PKC-mediated phosphorylation inhibition, astrocytosis, axonal proliferation, and microtubule assembly inhibition. S100B acts as a neurotrophic factor and neuronal survival protein in the developing CNS. |
