| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 21.1 kDa, a single non-glycosylated polypeptide chain containing 186 amino acids. |
| AA Sequence: |
MAPVLPGEDS KNVAAPHSQP LTSSERIDKH IRYILDGISA LRKETCNRSN MCESSKEALA ENNLNLPKMA EKDGCFQSGF NEDTCLVKII TGLLEFEVYL EYLQNRFESS EEQARAVQMS TKVLIQFLQK KAKNLDAITT PEPTTNASLL TKLQAQNQWL QDMTTHLILR SFKEFLQSNL RALRQM |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay usingIL-6-dependent murine 7TD1 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 × 107 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 50 mM Tris-HCl, pH9.0, 600 mM NaCl, with 0.02% Tween-20. |
| Endotoxin: |
Less than 1 EU/µg of rRhIL-6 as determined by LAL method. |
| Background: |
Interleukin-6 (IL-6) is a cytokine encoded by the IL-6 gene, functioning both as a pro-inflammatory and anti-inflammatory mediator. It is produced by T cells and macrophages to enhance immune responses. IL-6 is crucial for the maturation of B-cells into Ig-secreting cells, influencing lymphocyte and monocyte differentiation. Additionally, IL-6 promotes the growth of myeloma and plasmacytoma cells, and supports differentiation of nerve cells. Its effects span various cell types including B-cells, T-cells, hepatocytes, hematopoietic progenitor cells, and cells of the central nervous system (CNS). In rhesus macaques, IL-6 consists of 186 amino acids and signals through a receptor complex comprising the IL-6Rα chain (CD126) for ligand binding, and the signal-transducing component gp130 (CD130). |
