| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately12.6 kDa, a single non-glycosylated polypeptide chain containing 114 amino acids. |
| AA Sequence: |
SPSPVPRSTA LKELIEELVN ITQNQKAPLC NGSMVWSINL TAGVYCAALE SLINVSGCSA IEKTQRMLNG FCPHKVSAGQ FSSLRVRDTK IEVAQFVKDL LVHLKKLFRE GRFN |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 5 ng/mL, corresponding to a specific activity of > 2.0 × 105 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4, 3% trehalose. |
| Endotoxin: |
Less than 1 EU/μg of rRhIL-13 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Interleukin-13 (IL-13), produced by the IL13 gene, is secreted predominantly by Th2 cells. IL-13 is a monomer with two internal disulfide bonds, contributing to its four α-helix structure. Deleting IL-13 in mice impairs Th2 cell development and highlights IL-13's role in expelling gastrointestinal parasites. IL-13 has anti-inflammatory effects on monocytes and macrophages and inhibits the production of inflammatory cytokines like IL-1beta, TNF-alpha, IL-6, and IL-8. It also promotes B cell proliferation and IgE production through isotype switching. Mature rhesus IL-13 shares significant amino acid sequence identity with human, mouse, and rat IL-13. |
