| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 153 amino acids. |
| AA Sequence: |
APVRSLHCTL RDAQLKSLVM SGPYELKALH LQGQDLEQQV VFSMSFVQGE ESNDKIPVAL GLKAKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTRGG QDITDFTMQF VSS |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10.G4.1 cells is less than 10 pg/mL, corresponding to a specific activity of > 1.0 × 108 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rRhIL-1β as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Catabolin, Leukocyte Endogenous Mediator, LEM, Lymphocyte-activating factor , LAF, Mononuclear Cell Factor, MCF , Endogenous Pyrogen, EP |
| Background: |
Interleukin-1beta (IL-1β) is a non-secreted proinflammatory cytokine, mainly produced by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It performs metabolic, physiological, and haematopoietic activities, and plays a central role in regulating immune responses. IL-1α and IL-1β share the same receptor and have comparable, if not identical, biological properties. These cytokines stimulate thymocyte proliferation by inducing IL-2 release, promoting B-cell maturation and proliferation, exhibiting mitogenic FGF-like activity, and triggering the release of prostaglandin and collagenase from synovial cells. It's worth noting that the mature Rhesus macaque IL1β shows 96% amino acid sequence identity with rhesus IL1β, and 67%-78% with canine, mouse, and rat IL1β. |
