| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 8.7 kDa, a single non-glycosylated polypeptide chain containing 77 amino acids. |
| AA Sequence: |
IPLSRTVRCT CISISNQPVN PRSLEKLEII PPSQFCPHVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK ERSKRSP |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood T-lymphocytes is in a concentration range of 10-50 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rRhIP-10/CXCL10 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Gamma-IP10, Small-inducible Cytokine B10 |
| Background: |
CXCL10, also known as IP-10, belongs to the CXC chemokine family and is encoded by the CXCL10 gene. Initially identified in monocytes, endothelial cells, and fibroblasts, CXCL10 responds to IFN-γ stimulation. This chemokine exerts its effects by binding to the CXCR3 receptor on cell surfaces. CXCL10 serves as a chemoattractant for activated T-lymphocytes and monocytes/macrophages. It also plays roles in promoting T cell adhesion to endothelial cells and inhibiting bone marrow colony formation and angiogenesis. Rhesus macaque IP-10 shares sequence identities of 69%, 95%, and 74% with human, murine, and rat IP-10, respectively. |
