| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 11.5 kDa, a single non-glycosylated polypeptide chain containing 97 amino acids. |
| AA Sequence: |
SPNQEVARHH GDQHQAPRRW LWEGGQECDC KDWSLRVSKR KTTAVLEPPR KQCPCDHVKG SEKKNRRQKH HRKSQRPSRT CQQFLKRCQL ASFTLPL |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by its ability to induce VEGF expression using murine endothelial cells is less than 5.0 μg/mL, corresponding to a specific activity of > 200 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rRtVCC-1/CXCL17 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Dendritic Cell and Monocyte Chemokine-like Protein, DMC |
| Background: |
CXCL17, also known as Dendritic Cell and Monocyte Chemokine-like Protein (DMC) and VEGF Co-regulated Chemokine 1 (VCC-1), is a small cytokine in the CXC chemokine family. It is consistently expressed in the lungs and is also detectable in the trachea, stomach, and skeletal muscle. CXCL17 attracts dendritic cells and monocytes and is involved in tumor regulation. It likely functions as a housekeeping chemokine, managing the recruitment of nonactivated blood monocytes and immature dendritic cells into tissues, and may play a role in innate defense against infections. Mature human CXCL17 shares 73%, 71%, and 64% amino acid sequence identity with bovine, mouse, and rat DMC, respectively. |
