| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids. |
| AA Sequence: |
LRSSSQNSSD KPVAHVVANH QAEEQLEWLS QRANALLANG MDLKDNQLVV PADGLYLIYS QVLFKGQGCP DYVLLTHTVS RFAISYQEKV SLLSAIKSPC PKDTPEGAEL KPWYEPMYLG GVFQLEKGDL LSAEVNLPKY LDITESGQVY FGVIAL |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2.0 × 107 IU/mg in the presence of actinomycin D. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH7.2, 150 mM NaCl. |
| Endotoxin: |
Less than 1 EU/μg of rRtTNF-α as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing Factor , DIF, Necrosin, Cytotoxin |
| Background: |
Also known as cachectin, TNF-α is a prominent member of the TNF family and can induce cell death. It is synthesized by various cells, including neutrophils, activated lymphocytes, macrophages, and others. TNF-α exists in both secreted and membrane-bound forms, both active. The natural form is glycosylated, but recombinant non-glycosylated TNF-α is similarly active. Two receptor types for TNF-α have been identified, and most cell types express one or both types. |
