| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 12.5 kDa, a single non-glycosylated polypeptide chain containing 115 amino acids. |
| AA Sequence: |
MPMFIVNTNV PRASVPEGFL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF SGTSDPCALC SLHSIGKIGG AQNRNYSKLL CGLLSDRLHI SPDRVYINYY DMNAANVGWN GSTFA |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, pH8.0, 150 mM NaCl, 3% trehalose. |
| Endotoxin: |
Less than 1 EU/µg of rRtMIF as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
MIF, Glutathione-binding 13 kDa Protein, L-dopachrome Isomerase, L-dopachrome Tautomerase, Phenylpyruvate Tautomerase |
| Background: |
Macrophage migration inhibitory factor (MIF), also known as glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or phenylpyruvate tautomerase, is a protein encoded by the MIF gene. It is released from white blood cells in response to bacterial antigens to initiate an acute immune response, or in reaction to glucocorticoids to counteract their immune-suppressive effects. MIF forms a homotrimer, with each subunit consisting of 115 amino acids. Its primary functions include modulating the innate immune response against bacterial pathogens and neutralizing the anti-inflammatory actions of glucocorticoids. Additionally, MIF acts as a mediator in regulating macrophage function during host defense and exhibits phenylpyruvate tautomerase and dopachrome tautomerase activities in vitro. In terms of sequence similarity, rat MIF shares 99%, 90%, 89%, and 89% amino acid identity with its human, murine, porcine, and bovine counterparts, respectively. |
