| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids. |
| AA Sequence: |
QALGQDMVSP EATNSSSSSS SSSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using monkey 4MBr-5 cells is less than 120 ng/mL, corresponding to a specific activity of > 8.3 × 103 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, 500 mM NaCl, pH7.4, 5% trehalose. |
| Endotoxin: |
Less than 1 EU/µg of rRtKGF-2/FGF-10 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Fibroblast growth factor 10 (FGF-10) is a member of the FGF family, involved in embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. FGF-10, with a heparin-binding domain, is crucial for embryonic development regulation, cell proliferation, and differentiation. It also aids in wound healing and is necessary for normal branching morphogenesis. Recombinant rat FGF-10, with 179 amino acids, shares 94% and 100% amino acid sequence identity with human and murine FGF-10, respectively. |
