| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 26.2 kDa, a disulfide-linked homodimeric protein containing two 113 amino acids. |
| AA Sequence: |
MEIPMSTVVK ETLIQLSTHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEILFQNL SLIKKYIDGQ KEKCGEERRK TRHFLDYLQE FLGVMSTEWA MEV |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rRtIL-5 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
B-cell Differentiation Factor I, Eosinophil Differentiation Factor, TRF |
| Background: |
A cytokine family member, IL-5 is encoded near the genes for IL-3, IL-4, and GM-CSF, often co-expressed in Th2 cells. It binds to the IL-5 receptor, stimulating B cell growth and immunoglobulin secretion, and is a key mediator in eosinophil activation. IL-5 is associated with allergic diseases like allergic rhinitis and asthma. Rat IL-5, a 132-amino acid long Th2 cytokine, is a homodimer in its active form, unlike other cytokines in its family. |
