| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 17.4 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids. |
| AA Sequence: |
SIQGTSLLTE SCALSTYNDQ SVSFVLENGC YVINVEDCGK NQEKDKVLLR YYESSFPAQS GDGVDGKKLM VNMSPIKDTD IWLNANDKDY SVELQKGDVS PPDQAFFVLH KKSSDFVSFE CKNLPGTYIG VKDNQLALVE ENDESCNNIM FKLSKM |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH8.5. |
| Endotoxin: |
Less than 1 EU/µg of rRtIL-33 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
IL-33 |
| Background: |
Part of the IL-1 superfamily, IL-33, also known as NF-HEV and DVS 27, is a proinflammatory cytokine that may influence gene transcription and stimulates type 2 cytokine production from helper T cells, mast cells, eosinophils, and basophils. IL-33’s in vivo administration is linked to severe changes in mucosal organs, with constitutive expression in smooth muscle and airway epithelia and binding to the ST2 receptor. Recombinant rat IL-33, with 156 amino acids, shares 59% and 90% sequence identity with human and murine IL-33, respectively. |
