| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 15.2 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids. |
| AA Sequence: |
HKSSPQRPDH LLIRLRHLMD IVEQLKIYEN DLDPELLTAP QDVKGQCEHE AFACFQKAKL KPSNTGNNKT FINDLLAQLR RRLPAKRTGN KQRHMAKCPS CDLYEKKTPK EFLERLKWLL QKMIHQHLS |
| Purity: |
> 96% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human N1186 cells is less than 50 ng/mL, corresponding to a specific activity of > 2.0 × 104 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rRtIL-21 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Za11 |
| Background: |
Generated by CD4+ T cells upon antigenic stimulation, rat IL-21 regulates immune cells such as cytotoxic T cells and natural killer cells. Its effects include promoting the differentiation of B cells into plasma cells and memory B cells, stimulating IgG production with IL-4, and inducing apoptosis in B cells without T-cell signaling. It also enhances the anti-tumor activity of CD8+ T cells and NK cells. IL-21 operates through the IL-21R, which includes the gamma chain found in other cytokine receptors. Mature rat IL-21 shares 88% amino acid sequence identity with mouse IL-21 and is associated with various clinical conditions, including cancer immunotherapy, viral infections, and allergies. |
