| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 135 amino acids. |
| AA Sequence: |
APTSSPAKET QQHLEQLLLD LQVLLRGIDN YKNLKLPMML TFKFYLPKQA TELKHLQCLE NELGALQRVL DLTQSKSFHL EDAGNFISNI RVTVVKLKGS ENKFECQFDD EPATVVEFLR RWIAICQSII STMTQ |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/mL, corresponding to a specific activity of > 5.0 × 106 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
A 0.2 μm filtered concentrated sterile solution in 10 mM sodium citrate, pH4.0. |
| Endotoxin: |
Less than 1 EU/μg of rRtIL-2 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
T-cell Growth Factor, CGF |
| Background: |
IL-2 is a potent immunoregulatory molecule generated by T-cells upon stimulation. It is found in various immune cells, including CD4+ and CD8+ T cells, and is essential for T-cell proliferation and vital immune functions. The IL-2 receptor is composed of three subunits and exists in different complexes on the cell surface. Recombinant rat IL-2, a 15.3 kDa protein with 134 amino acids, shares varying degrees of sequence identity with IL-2 from other species, including mice, humans, and others. |
