| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 29.8 kDa, a homodimeric protein consisting of two 134 amino acid non-glycosylated polypeptide chains. |
| AA Sequence: |
SPDKQAAALP RRERNRQAAA ASPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCEAAETMYD KILKNLSRSR RLTSDKVGQA CCRPVAFDDD LSFLDDSLVY HILRKHSAKR CGCI |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat C6 cells is less than 0.2 ng/mL, corresponding to a specific activity of > 5.0 × 106 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH7.4. |
| Endotoxin: |
Less than 0.1 EU/μg of rRtGDNF as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
ATF |
| Background: |
Glial cell-derived neurotrophic factor (GDNF) is a key member of the GDNF family of ligands and interacts with GFRA2 and GDNF family receptor alpha 1. This compact protein significantly enhances the survival and morphological differentiation of various neurons and may influence neuronal activity in motor neuron's distal regions. The recombinant rat GDNF, a monomeric form, comprises 135 amino acid residues forming a disulfide-linked homodimer, sharing 99% and 93% amino acid sequence identity with its mouse and human counterparts, respectively. |
