| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 23.1 kDa, a single non-glycosylated polypeptide chain containing 205 amino acids. |
| AA Sequence: |
LGEVGSYFGV QDAVPFGNVP VLPVDSPVLL SDHLGQSEAG GLPRGPAVTD LDHLKGILRR RQLYCRTGFH LEIFPNGTIQ GTRKDHSRFG ILEFISIAVG LVSIRGVDSG LYLGMNEKGE LYGSEKLTQE CVFREQFEEN WYNTYSSNLY KHVDTGRRYY VALNKDGTPR EGTRTKRHQK FTHFLPRPVD PDKVPELYKD ILSQS |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, 400 mM NaCl, pH8.0. |
| Endotoxin: |
Less than 1 EU/µg of rRtFGF-9-1 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
GAF, Glia-activating factor, HBGF-9 |
| Background: |
Belonging to the fibroblast growth factor family, FGF-9 is a heparin-binding protein with a core domain of 120 amino acids that confers a shared tertiary structure among FGFs. It is pivotal in embryonic development, cellular growth, differentiation, and migration. Identified as a growth-promoting secreted factor for glial cells in culture, FGF-9 functions as a monomer and interacts with receptors FGFR1 through FGFR4. Rat FGF-9 exhibits a 98% amino acid sequence similarity with its human counterpart. |
