| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 6.1 kDa, a single non-glycosylated polypeptide chain containing 53 amino acids, including 3 intramolecular disulfide-bonds. |
| AA Sequence: |
NSNTGCPPSY DGYCLNGGVC MYVESVDRYV CNCVIGYIGE RCQHRDLRWW KLR |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/mL, corresponding to a specific activity of > 1.0 × 107 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rRtEGF as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Urogastrone, URG |
| Background: |
Epidermal Growth Factor (EGF) was first identified in nerve growth factor preparations from mouse submaxillary glands, causing early eyelid opening, incisor eruption, hair growth inhibition, and growth stunting in newborn mice. It is the prototype of a family of growth factors derived from membrane-anchored precursors, all featuring at least one EGF structural unit in their extracellular domain. EGF is initially produced as a 130 kDa transmembrane precursor with 9 EGF units, with the mature soluble EGF corresponding to the unit closest to the transmembrane domain. This precursor can bind to the EGF receptor and is biologically active. Mature rat EGF shares 70% amino acid sequence identity with human EGF. |
