| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids. |
| AA Sequence: |
RELRCQCLKT LPRVDFENIQ SLTVTPPGPH CTQTEVIATL KDGQEVCLNP QAPRLQKIIQ KLLKSDKSS |
| Purity: |
> 96% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 5-50 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH7.4, 50 mM NaCl. |
| Endotoxin: |
Less than 1 EU/μg of rRtCINC-2α/CXCL3 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
CINC-2alpha, MIP2-alpha |
| Background: |
CXCL3, a member of the CXC chemokine family, is also known as CINC-2 in rats, DCIP-1 in mice, and GROγ in humans. Its functional receptor is identified as CXCR2. Like other GRO proteins, CXCL3 is a potent attractant and activator of neutrophils. This chemokine was initially purified from the conditioned medium of rat granulation tissue. In rats, CINC-2 exists in two isoforms: CXCL3α (CINC-2α) and CXCL3β (CINC-2β). The difference between CXCL3α and CXCL3β lies in their carboxy-terminal residues, with CXCL3α having DKSS and CXCL3β having PSL at positions 98-101. |
