| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 7.8 kDa, a single, non-glycosylated polypeptide chain containing 72 amino acids. |
| AA Sequence: |
APVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREA CLDPEAPMVQ KIVQKMLKGV PK |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using rat neutrophils is in a concentration range of 10-100 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH7.4, 150 mM NaCl. |
| Endotoxin: |
Less than 1 EU/μg of rRtCINC-1/CXCL1 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
GRO-alpha, Platelet-derived Growth Factor-inducible Protein KC |
| Background: |
Rat CXCL1, also known as CINC-1, belongs to the CXC chemokine family and is encoded by the CXCL1 gene, formerly known as GRO. Originally isolated from media conditioned by IL-1β stimulated rat kidney epithelioid cells, CINC-1 is a member of the intercrine alpha (chemokine C-X-C) subfamily. It is secreted by human melanoma cells, as well as expressed by macrophages, neutrophils, and epithelial cells. The functional receptor for CXCL1 is CXCR2. CXCL1 exhibits chemotactic activity for neutrophils and plays a role in inflammation and wound healing. The amino acid sequence of rat CXCL1 shares approximately 68% identity with human CXCL1. |
