| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 9.1 kDa, a single non-glycosylated polypeptide chain containing 78 amino acids. |
| AA Sequence: |
ARVSAELRCQ CINTHSTPFH PKFIKELRVI ESGPHCENSE IIVKLVNGKE VCLDPKEKWV QKVVQIFLKR TEKQQQQQ |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 20-200 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rPoIL-8/CXCL8 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
(Ser-IL-8)72, GCP/IL-8 Protein I, IL8/NAP1 form III, LYNAP, MDNCF-c, NAF |
| Background: |
Interleukin-8 (IL-8), encoded by the IL8 gene, is produced by macrophages and other cells like epithelial cells. Endothelial cells also synthesize IL-8, storing it in vesicles. Several receptors can bind IL-8, with CXCR1 and CXCR2 showing the highest affinity. As a CXC chemokine family member, IL-8 induces chemotaxis in target cells such as neutrophil granulocytes, basophils, and T-cells. It is often linked to inflammation and cited as a proinflammatory mediator in conditions like gingivitis and psoriasis. |
