| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids. |
| AA Sequence: |
ARATNVGREC CLDYFKGAIP IRKLVSWYKT SVECSRDAIV FLTVQGKLIC ADPKDKHVKK AIRLVKNPRP |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rMuTARC/CCL17 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Murine CCL17, also known as TARC (Thymus and activation-regulated chemokine), Small-inducible cytokine A17, and SCYA17, is encoded by the CCL17 gene situated on chromosome 8. Within the CC chemokine family, CCL17 shares approximately 24% to 29% amino acid sequence identity with RANTES, MIP-1α, MIP-1β, MCP-1, MCP-2, MCP-3, and I-309. It is a secreted protein expressed constitutively by thymus cells and transiently by phytohemagglutinin-stimulated peripheral blood mononuclear cells. CCL17 exerts its effects through chemokine receptors CCR4 and CCR8, demonstrating chemotactic activity towards T lymphocytes and certain other leukocytes.
CCL17 plays a significant role in skin disorders such as atopic dermatitis, bullous pemphigoid, and mycosis fungoides. Recombinant CCL17 has been demonstrated to be chemotactic for T cell lines but does not exhibit such activity towards monocytes or neutrophils. |
