| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 10.7 kDa, a single non-glycosylated polypeptide chain containing 95 amino acids. |
| AA Sequence: |
GLIQEIEKED RRYNPPIIHQ GFQDTSSDCC FSYATQIPCK RFIYYFPTSG GCIKPGIIFI SRRGTQVCAD PSDRRVQRCL STLKQGPRSG NKVIA |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human CCR1 transfected murine BaF3 cells is in a concentration range of 10-100 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, pH8.0, 500 mM NaCl. |
| Endotoxin: |
Less than 1 EU/µg of rMuC10/CCL6 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Small-inducible Cytokine A6 |
| Background: |
Chemokine (C-C motif) ligand 6 (CCL6), encoded by the CCL6 gene, is a small cytokine of the CC chemokine family, identified exclusively in rodents. In murine models, CCL6 expression is observed in myelopoietic bone marrow cultures stimulated by GM-CSF, M-CSF, IL-3, or IL-4. It has also been found in IL-2-dependent T cell lines, with expression inhibited upon T cell activation. CCL6 primarily signals through the CCR1 receptor. Functionally, CCL6 acts as a chemoattractant for B cells, CD4+ T cells, monocytes, and NK cells. Moreover, it exerts potent suppressive effects on colony formation by various types of hematopoietic progenitor cells. CCL6, like other CC chemokines, contains four highly conserved cysteine residues in its structure (C10 motif). |
