| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 8.2 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids. |
| AA Sequence: |
VTSAGPEESD GDLSCVCVKT ISSGIHLKHI TSLEVIKAGR HCAVPQLIAT LKNGRKICLD RQAPLYKKVI KKILES |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 1.5 M NaCl, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rMuPF-4/CXCL4 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
CXCL4, also known as platelet factor 4 (PF4), is a small cytokine belonging to the CXC chemokine family. This chemokine is released from alpha-granules of activated platelets during platelet aggregation. CXCL4 plays a crucial role in neutralizing the anticoagulant effect of heparin by binding more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. It interacts with a splice variant of the chemokine receptor CXCR3. Recombinant mouse CXCL4 consists of 76 amino acids and is a single non-glycosylated polypeptide chain. Human and mouse CXCL4 share approximately 60% sequence identity. |
