| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 182 amino acids. |
| AA Sequence: |
APISPSEPIG QAYSLALYMQ KNTSALLQTY LQHQGSPFSD PGFSAPELQL STLPSAAVSF KTWHAMEDAE RLSRAQGAFL ALTQHLQLVG DDQSYLNPGS PILLAQLGAA RLRAQGLLGN MAAIMTALGL PIPPEEDTLG FVPFGASAFE RKCRGYIVTR EYGHWTDRAV RDLALLKAKY SA |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 200 ng/mL, corresponding to a specific activity of > 5000 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris-HCl, pH8.0, 500 mM NaCl, with 0.5 mM DTT. |
| Endotoxin: |
Less than 1 EU/μg of rMuNeuropoietin as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Ctf2 |
| Background: |
Neuropoietin (NP), also referred to as cardiotrophin-2, belongs to the IL-6 family of cytokines with a molecular weight of 19.7 kDa. It emerged from a gene duplication event involving cardiotrophin-1 (CT-1), defining a distinct subgroup alongside CT-1, CLC, and CTNF within the IL-6 family. In mice, neuropoietin is synthesized as a precursor of 204 amino acids (aa), featuring a 22 aa signal sequence and a mature segment of 182 aa. The mature protein exhibits four alpha-helices, characteristic of hematopoietic superfamily molecules. Across species, mature murine neuropoietin shares significant amino acid sequence identities of 88% with chimpanzees, 90% with dogs, and 95% with rats. |
