| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 9.2 kDa, a single non-glycosylated polypeptide chain containing 83 amino acids. |
| AA Sequence: |
GANDAEDCCL SVTQRPIPGN IVKAFRYLLN EDGCRVPAVV FTTLRGYQLC APPDQPWVDR IIRRLKKSSA KNKGNSTRRS PVS |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human mature dendritic cells is in a concentration range of 10-100 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rMuMIP-3β/CCL19 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Beta-chemokine Dxodus-3, CK beta-11, EBI1 Ligand Dhemokine, ELCMacrophage Inflammatory Protein 3 beta |
| Background: |
CCL19, alternatively known as EBI1 ligand chemokine (ELC) and macrophage inflammatory protein-3-beta, is produced from the CCL19 gene found on chromosome 4 in mice. It is notably expressed at significant levels in the thymus and lymph nodes, moderately in the trachea and colon, and minimally in organs like the stomach, lungs, and others. CCL19 exerts its effects by signaling through CCR7, facilitating the chemotaxis of T and B lymphocytes, as well as myeloid progenitor cells. |
