| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 8.0 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids. |
| AA Sequence: |
ASNYDCCLSY IQTPLPSRAI VGFTRQMADE ACDINAIIFH TKKRKSVCAD PKQNWVKRAV NLLSLRVKKM |
| Purity: |
> 96% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human CCR6 transfected murine BaF3 cells is in a concentration range of 0.1-10 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rMuMIP-3α/CCL20 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
LARC, Beta-chemokine Dxodus-1, Liver and Activation-regulated Chemokine |
| Background: |
CCL20, also known as liver and activation-regulated chemokine (LARC), is encoded by the CCL20 gene located on chromosome 1 in mice. Its expression can be induced by lipopolysaccharide (LPS) and certain inflammatory cytokines, and it is highly expressed in lymph nodes, peripheral blood leukocytes (PBL), liver, lung, and appendix. CCL20 functions as a chemotactic agent for lymphocytes and dendritic cells. Moreover, it enhances the adhesion of memory CD4+ T cells and suppresses colony formation of immature myeloid progenitors in the bone marrow. CCL20 exerts its effects by binding to the CCR6 receptor. |
