| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately19.5 kDa, a single non-glycosylated polypeptide chain containing 173 amino acids. |
| AA Sequence: |
QALGQDMVSQ EATNCSSSSS SFSSPSSAGR HVRSYNHLQG DVRWRRLFSF TKYFLTIEKN GKVSGTKNED CPYSVLEITS VEIGVVAVKA INSNYYLAMN KKGKLYGSKE FNNDCKLKER IEENGYNTYA SFNWQHNGRQ MYVALNGKGA PRRGQKTRRK NTSAHFLPMT IQT |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/mL, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, 600 mM NaCl, pH7.4, 1 mM mercaptoethanol. |
| Endotoxin: |
Less than 1 EU/µg of rMuKGF-2/FGF-10 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
The fibroblast growth factor (FGF) family includes Fibroblast growth factor 10, which plays a role in various biological processes such as embryonic development, tissue repair, morphogenesis, cell growth, tumor invasion, and growth. Like most other FGF family members, FGF-10 contains a heparin-binding domain playing a vital role in embryonic development, cell proliferation, and differentiation regulation. Furthermore, FGF-10 is required for normal branching morphogenesis, possibly participating in wound healing. The recombinant murine FGF-10 consists of 173 amino acids and shares a 94% and 100% a.a. sequence identity with human and rat FGF-10 respectively. |
