| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 18.7 kDa, a single, non-glycosylated polypeptide chain containing 163 amino acids. |
| AA Sequence: |
CNDMSPEQTA TSVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNSYNIMEIR TVAVGIVAIK GVESEYYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHSGG EMFVALNQKG IPVKGKKTKK EQKTAHFLPM AIT |
| Purity: |
> 96% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10 ng/mL, corresponding to a specific activity of > 1.0 × 105 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered solution in 20 mM PB, pH8.0, 1 M NaCl. |
| Endotoxin: |
Less than 1 EU/µg of rMuKGF-1/FGF-7 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
,HBGF-7 |
| Background: |
Murine KGF-1, also known as Fibroblast growth factor 7 (FGF-7), is manufactured by the FGF7 gene. KGF-1 only binds to the b splice form of FGFR2b/KGFR, the tyrosine kinase receptor. The affinity between KGF-1 and its receptor can be increased by heparin or heparan sulfate proteoglycan. FGF-10, also known as keratinocyte growth factor 2 (KGF-2), shares a 51% amino acid sequence identity with KGF-1, along with exhibiting similar functions but uses an additional receptor, FGFR2c. Murine KGF-1 shares 96% of the human and rat a.a. sequence identity. |
