| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 72 amino acid residues. |
| AA Sequence: |
APIANELRCQ CLQTMAGIHL KNIQSLKVLP SGPHCTQTEV IATLKNGREA CLDPEAPLVQ KIVQKMLKGV PK |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 10-100 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rMuKC/CXCL1 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
C-X-C motif chemokine 1, GRO-alpha, Platelet-derived growth factor-inducible protein, KC, Secretory protein N51 |
| Background: |
Murine CXCL1, also known as KC, belongs to the CXC chemokine family and is encoded by the CXCL1 gene, previously designated as GRO. Initially discovered in mouse fibroblasts stimulated by platelet-derived growth factor, KC is a member of the CXC chemokine subfamily. It is secreted by human melanoma cells and expressed by macrophages, neutrophils, and epithelial cells. The functional receptor for CXCL1 is CXCR2. CXCL1 exhibits chemotactic activity for neutrophils and plays a crucial role in inflammation and wound healing. The amino acid sequence of murine CXCL1 shares approximately 60% identity with human CXCL1. KC has been implicated in monocyte adhesion to atherosclerotic endothelium and may also contribute to the pathophysiology of Alzheimer’s disease. |
