| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 21.7 kDa, a single non-glycosylated polypeptide chain containing 188 amino acids. |
| AA Sequence: |
MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6-dependent murine 7TD1 cells is less than 0.02 ng/mL, corresponding to a specific activity of > 5 × 107 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered solution in 30 mM Acetic Acid, pH3.0, 150 mM NaCl, 5% Trehalose, 0.02% Tween-20. |
| Endotoxin: |
Less than 1 EU/µg of rMuIL-6 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
B-cell hybridoma growth factor, Interleukin HP-1. |
| Background: |
Interleukin-6 (IL-6), a cytokine with pro-inflammatory and anti-inflammatory properties, is encoded by the IL-6 gene in humans. Immune response stimulation is triggered when it is secreted by T cells and macrophages. It plays a vital role in the final differentiation of B-cells into Ig-secreting cells, which are involved in the differentiation of lymphocytes and monocytes. Specifically, IL-6 promotes the growth of myeloma and plasmacytoma, stimulates nerve cell differentiation, and has actions on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells, and CNS cells. The mouse variant of IL-6 is a single, non-glycosylated polypeptide chain that contains 187 amino acids. It signals through a cell-surface type I cytokine receptor complex that consists of the ligand-binding IL-6Rα chain (CD126) and the signal-transducing component gp130 (also known as CD130). Sequence identity of approximately 40% and 85% is shared with human and rat IL-6 respectively, and it is just as active on human and rat cells. |
