| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 16.9 kDa, a single non-glycosylated polypeptide chain containing 154 amino acids. |
| AA Sequence: |
VLSGALCFRM KDSALKVLYL HNNQLLAGGL HAEKVIKGEE ISVVPNRALD ASLSPVILGV QGGSQCLSCG TEKGPILKLE PVNIMELYLG AKESKSFTFY RRDMGLTSSF ESAAYPGWFL CTSPEADQPV RLTQIPEDPA WDAPITDFYF QQCD |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Measured by its ability to inhibit IL-36γ induced IL-6 production by human PBMCs. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rMuIL-36RA as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
IL-1HY1, IL-1 delta, IL-1F5, IL-1H3, IL-1L1 |
| Background: |
Interleukin-36 receptor antagonist (IL-36RA) is a part of the IL-1 family and is a secreted protein that primarily expressed in keratinocytes, not including fibroblasts, endothelial cells, or melanocytes. IL-36RA has been suggested to act as an antagonist to IL-36α (IL-1F6), IL-36β (IL-1F8), and IL-36γ (IL-1F9). Moreover, IL-36RA could form part of a signaling system that is independent, similarly to interleukin-1 alpha (IL-1A), beta (IL-1B) receptor agonist, and interleukin-1 receptor type I (IL-1R1). Still, the definite receptor for IL-36RA remains unidentified, with indirect evidence pointing towards IL-1Rrp2. Recombinant murine interleukin-36 RA consists of 154 amino acid residues and shares 91% a.a. sequence identity with human IL-36RA. |
