| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 14.8 kDa globular protein containing 134 amino acid residues. |
| AA Sequence: |
DTHRLTRTLN CSSIVKEIIG KLPEPELKTD DEGPSLRNKS FRRVNLSKFV ESQGEVDPED RYVIKSNLQK LNCCLPTSAN DSALPGVFIR DLDDFRKKLR FYMVHLNDLE TVLTSRPPQP ASGSVSPNRG TVEC |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is less than 0.05 ng/mL, corresponding to a specific activity of > 2 × 107 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rMuIL-3 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Hematopoietic growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor. |
| Background: |
IL-3, a cytokine encoded by the IL-3 gene on chromosome 5, is primarily produced by activated T cells, as well as human thymic epithelial cells, activated murine mast cells, murine keratinocytes, and neurons/astrocytes. It regulates hematopoiesis by controlling the production, differentiation, and function of granulocytes and monocytes-macrophages. IL-3 acts through receptors composed of α and β subunits. Mouse IL-3, containing 140 amino acids, shares low homology with human IL-3 and lacks cross-species activity. |
