| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 149 amino acids. |
| AA Sequence: |
APTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ LLMDLQELLS RMENYRNLKL PRMLTFKFYL PKQATELKDL QCLEDELGPL RHVLDLTQSK SFQLEDAENF ISNIRVTVVK LKGSDNTFEC QFDDESATVV DFLRRWIAFC QSIISTSPQ |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/mL, corresponding to a specific activity of > 5.0 × 106 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rMuIL-2 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
T-cell growth factor, TCGF, Aldesleukin |
| Background: |
IL-2, a potent immunoregulatory lymphokine produced by T-cells upon antigenic or mitogenic stimulation, is expressed by various immune cells. IL-2/IL-2R signaling is essential for T-cell proliferation and other critical immune functions. The IL-2 receptor comprises three subunits: IL2Rα, IL2Rβ, and the common gamma chain γc/IL2Rγ. Mature mouse IL-2 shares 56% and 73% amino acid sequence identity with human and rat IL-2, respectively, and has cross-species activity. Mouse IL-2 features a unique N-terminal region with a polyglutamine stretch. |
