| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 12.2 kDa, a single non-glycosylated polypeptide chain containing 118 amino acids. |
| AA Sequence: |
SAASASAASD ISVESKEATV CTVTLEKTSA GLGFSLEGGK GSLHGDKPLT INRIFKGTEQ GEMVQPGDEI LQLAGTAVQG LTRFEAWNVI KALPDGPVTI VIRRTSLQCK QTTASADS |
| Purity: |
> 98% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral T lymphocytes is in a concentration range of 1.0-100 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rMuIL-16 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Lymphocyte chemoattractant factor , LCF |
| Background: |
Interleukin-16, also known as lymphocyte chemoattractant factor (LCF), is predominantly secreted by lymphocytes, epithelial cells, eosinophils, and CD8+ T cells. Its functions are diverse, including the induction of IL-2Rα on T cells, suppression of HIV replication, and inhibition of T-cell antigen receptor/CD3 mediated stimulation in mixed lymphocyte reactions, among others. It operates through the CD4 receptor. Recombinant murine IL-16 comprises 118 amino acids and shares approximately 85% sequence identity with various human IL-16 isoforms. |
