| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 12.3 kDa, a single non-glycosylated polypeptide chain containing 111 amino acids. |
| AA Sequence: |
MPVPRSVSLP LTLKELIEEL SNITQDQTPL CNGSMVWSVD LAAGGFCVAL DSLTNISNCN AIYRTQRILH GLCNRKAPTT VSSLPDTKIE VAHFITKLLS YTKQLFRHGP F |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 4 ng/mL, corresponding to a specific activity of > 2.5 × 105 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rMuIL-13 as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Background: |
Murine IL-13, encoded by the IL13 gene on chromosome 11, is secreted by several cell types, notably T helper type 2 (Th2) cells. The targeted deletion of IL-13 in mice has shown its crucial role in Th2 cell development and in the expulsion of gastrointestinal parasites. IL-13 is also implicated in asthma, allergy, atopy, and inflammatory responses, making it a significant therapeutic target. Human, mouse, and rat IL-13 exhibit low homology but are capable of cross-species activity. |
