| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 17.5 kDa, a single non-glycosylated polypeptide chain containing 152 amino acids. |
| AA Sequence: |
VPIRQLHYRL RDEQQKSLVL SDPYELKALH LNGQNINQQV IFSMSFVQGE PSNDKIPVAL GLKGKNLYLS CVMKDGTPTL QLESVDPKQY PKKKMEKRFV FNKIEVKSKV EFESAEFPNW YISTSQAEHK PVFLGNNSGQ DIIDFTMESV SS |
| Purity: |
> 96% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/mL, corresponding to a specific activity of > 5.0 × 108 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered solution in PBS, pH7.4, with 5% trehalose, 0.02% Tween-20. |
| Endotoxin: |
Less than 1 EU/µg of rMuIL-1β as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Catabolin, Leukocyte Endogenous Mediator, LEM, Lymphocyte-activating factor , LAF, Mononuclear Cell Factor, MCF , Endogenous Pyrogen, EP |
| Background: |
IL-1β, a proinflammatory cytokine not secreted but produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells, has various metabolic, physiological, and hematopoietic activities and is central to immune response regulation. IL-1α and IL-1β bind to the same receptor and have similar, though not identical, effects. Mature mouse IL-1β shares 90% amino acid sequence identity with cotton rat and rat IL-1β and 65%-78% with canine, human, and rhesus IL-1β. |
