| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 17.9 kDa, a single non-glycosylated polypeptide chain containing 156 amino acids. |
| AA Sequence: |
SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR VHLARGLPSM TDFQIS |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 2 pg/mL, corresponding to a specific activity of > 5.0 × 108 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered solution in PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rMuIL-1α as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Lymphocyte-activating factor, LAF, Leukocyte Endogenous Mediator, LEM, Endogenous Pyrogen, EP, Mononuclear Cell Factor, MCF, Hematopoietin-1 |
| Background: |
IL-1α, like IL-1β, is a proinflammatory cytokine produced mainly by activated macrophages and other cell types. It has similar metabolic, physiological, and hematopoietic activities and plays a central role in immune response regulation. IL-1α and IL-1β bind to the same receptor and have similar effects. The amino acid sequence of mature IL-1α is conserved 60%-70% across various species, and human IL-1α is biologically active on murine cell lines. |
