| Source: |
Escherichia coli. |
| Molecular Weight: |
Approximately 9.8 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids. |
| AA Sequence: |
DLEGTDLNLF KVAFSSKPQG LATPSKERNG KKKKKGKGLG KKRDPCLRKY KDYCIHGECR YLQEFRTPSC KCLPGYHGHR CHGLTL |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 1 ng/mL, corresponding to a specific activity of > 1.0 × 106 IU/mg. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, 500 mM NaCl, pH7.4. |
| Endotoxin: |
Less than 1 EU/μg of rMuHB-EGF as determined by LAL method. |
| Reconstitution: |
Centrifuge the vial briefly before opening to ensure that the contents settle at the bottom. Reconstitute the vial with sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide the resulting stock solution into working aliquots and store them at or below -20°C. For further dilutions, use appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
HBEGF, DT-R |
| Background: |
Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is present in cerebral neurons and its expression escalates following hypoxic or ischemic injury, which also promotes neurogenesis. HB-EGF is involved in various normal physiological processes, including blastocyst implantation and wound healing, as well as pathological processes like tumor growth, smooth muscle cell (SMC) hyperplasia, and atherosclerosis. This 87 amino acid mitogenic and chemotactic glycoprotein features an EGF-like domain with six conserved cysteine residues. The mouse HB-EGF exhibits approximately 73% amino acid sequence identity with the human variant. |
