| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 8.7 kDa, a single non-glycosylated polypeptide chain containing 77 amino acids. |
| AA Sequence: |
IPLARTVRCN CIHIDDGPVR MRAIGKLEII PASLSCPRVE IIATMKKNDE QRCLNPESKT IKNLMKAFSQ KRSKRAP |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human peripheral blood lymphocytes is in a concentration range of 0.1-10.0 ng/mL in the presence of IL-2. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH7.4. |
| Endotoxin: |
Less than 1 EU/µg of rMuIP-10/CXCL10 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
Gamma-IP10, Small-inducible Cytokine B10, C7, Interferon-gamma Induced Protein CRG-2 |
| Background: |
CXCL10, also known as IP-10, belongs to the CXC chemokine family and is encoded by the CXCL10 gene, also termed CRG-2 in mice. Initially identified as an immediate early gene in response to macrophage activation, CXCL10 exerts its effects by binding to the chemokine receptor CXCR3 on cell surfaces. This chemokine functions as a chemoattractant for activated T-lymphocytes and monocytes/macrophages. Additionally, CXCL10 plays roles in promoting T cell adhesion to endothelial cells, and in inhibiting bone marrow colony formation and angiogenesis. Murine CXCL10 shares approximately 67% amino acid sequence identity with its human counterpart. |
