| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 14.2 kDa, a single non-glycosylated polypeptide chain containing 127 amino acids. |
| AA Sequence: |
MNFSGKYQLQ SQENFEPFMK AIGLPEDLIQ KGKDIKGVSE IVHEGKKIKL TITYGPKVVR NEFTLGEECE LETMTGEKVK AVVKLEGDNK MVTTFKGIKS VTELNGDTIT NTMTLGDIVY KRVSKRI |
| Purity: |
> 95% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The binding affinity of rMuFABP1 for the synthetic ligand cis-parinaric acid has been measured by fluorescence titration. Half maximal fluorescence of 2.5 μM rMuFABP1 is achieved with approximately 5 μM cis-paranaric acid. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4, 2% trehalose. |
| Endotoxin: |
Less than 1 EU/µg of rMuFABP1 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
14 kDa Selenium-binding Protein, Fatty Acid-binding Protein 1, L-FABP |
| Background: |
Fatty acid-binding proteins (FABPs) are a group of carrier proteins responsible for transporting fatty acids and other lipophilic substances like eicosanoids and retinoids. They play a crucial role in transferring fatty acids between cellular membranes. Fatty acid-binding protein 1 (FABP1), also known as liver-type fatty acid-binding protein (L-FABP), is a member of this family encoded by the FABP1 gene. It is a small, highly conserved cytoplasmic protein. FABP1 binds not only free fatty acids and their coenzyme A derivatives but also bilirubin and various other small molecules within the cytoplasm, potentially facilitating intracellular lipid transport. In terms of sequence similarity, murine FABP1 shares 84% and 94% amino acid sequence identity with its human and rat counterparts, respectively. |
