| Source: |
Escherichia coli |
| Molecular Weight: |
Approximately 12.1 kDa, a single, non-glycosylated polypeptide chain containing 110 amino acids. |
| AA Sequence: |
SDGGGQDCCL KYSQKKIPYS IVRGYRKQEP SLGCPIPAIL FLPRKHSKPE LCANPEEGWV QNLMRRLDQP PAPGKQSPGC RKNRGTSKSG KKGKGSKGCK RTEQTQPSRG |
| Purity: |
> 97% by SDS-PAGE and HPLC analyses. |
| Biological Activity: |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine T-lymphocytes is in a concentration range of 10-100 ng/mL. |
| Physical Appearance: |
Sterile filtered white lyophilized (freeze-dried) powder. |
| Formulation: |
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH7.4, 150 mM NaCl. |
| Endotoxin: |
Less than 1 EU/μg of rMuExodus-2/CCL21 as determined by LAL method. |
| Reconstitution: |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage: |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70°C as supplied.
1 month, 2 to 8°C under sterile conditions after reconstitution.
3 months, -20 to -70°C under sterile conditions after reconstitution. |
| Synonyms: |
6Ckine, Beta-chemokine Exodus-2, SLC |
| Background: |
CCL21, encoded by the CCL21 gene located on Chromosome 4, shares 21-33% sequence identity with other CC chemokines. This chemokine includes the four conserved cysteines typical of β chemokines, along with two additional cysteines in its unusually long carboxyl-terminal domain. Human and mouse CCL21 are highly similar, sharing 86% amino acid sequence identity. CCL21 is prominently expressed in lymph nodes, specific endothelial cells, as well as in the spleen and appendix. It exerts its effects by binding to CCR7, facilitating chemotaxis of T and B lymphocytes, and also plays a role in inhibiting hematopoiesis. |
